Nematode Lab


solid tests & reliable results

the measures that help you level-up your impact

Columbia Root-knot Nematode

Northern Root-knot Nematode

Stubby Root Nematode

Root Lesion Nematode

Stem & Bulb Nematode

Dagger Nematode

Ring Nematode

Stunt Nematode

Spiral Nematode

Pin Nematode

Soil, Roots, and Tuber samples

Plant-parasitic nematode testing

Test / Description

N1 Nematode Assay - Soil; Nematode Composition, Genera Identification & Count

N2 Nematode Assay for Potato Fields - Soil; Nematode Composition, Genera/Species Identification & Count (including chitwoodi and hapla)

N3 Nematode Assay - Roots/Plant materials; Nematode Composition, Genera Identification & Count

N4 Nematode Assay - Irrigation Water sample – Nematode Composition, Genera Identification & Count

N5 Extras: Nematode Species Identification

N6 Cyst Extraction & Count

N7 Potato tuber analysis for root-knot nematode infection using staining and imaging techniques

N8 Onion/Garlic/Tulip Nematode Assay - Bulbs; Stem & Bulb nematode (Ditylenchus dipsaci)

N9 Nematode Examination on roots sample using staining and imaging techniques

N10 DNA genetic testing & analysis - Columbia (M. chitwoodi) & Northern (M. hapla) root-knot nematodes

N11 DNA genetic testing & analysis - RKNs and SRN

N12 DNA genetic testing & analysis - Testing Stubby Root Nematodes (SRN) for Tobacco Rattle Virus (TRV)

N13 Extras for N1/N2: Soil Nematode Activity for Soil Health Assessment (including Saprophytic & Beneficial nematodes) – population count

Nematode Testing Service

Intro: Nematodes are important economic pests of all types of crops. These pests are particularly problematic in commercial farms. Nematodes are microscopic, unsegmented roundworms that live in the soil and roots system. All parasitic nematodes have a needle-like mouthpart (stylet) used to puncture the plant cell, inject digestive juices, and ingest plant fluids.

Disease complex: In addition to direct damage, nematodes may also enhance crop damage by fungal pathogens. Nematode feeding may aid fungal infection and development and increase the level of damage that is caused. Soil fungal pathogens known to form disease complexes includes but not limited to Verticillium, Fusarium, Rhizoctonia and Pythium species.

Virus Transmission: Some nematodes transmit viruses while feeding on roots, the consequence of viral diseases evaluates much higher than their direct yield loss. Some of viral diseases known to transmit by nematodes are Tobacco Rattle Virus (TRV), Cherry Raspleaf Virus (CRLV), Tobacco Ringspot Virus (TRSV), Tomato Ringspot Virus (ToRSV), and Grape fanleaf Virus (GFLV). Therefore, nematode testing for presence/absence of viruses can help prevent spread of viral diseases [AGNEMA has improved assays and made them available for commercial purposes].

Diagnosis: Nematode problems are often misdiagnosed as being the result of poor cultural practices, diseases, insect damage, soil compaction, nutrient deficiencies, poor drainage, drought or other environmental stresses. To accurately diagnose a nematode problem, a soil sample must be collected from the affected area and be assayed by a Nematology laboratory where trained professionals can determine if there are parasitic nematodes present at levels that could cause the observed damages.

Economic impact: In the pacific northwest of the united states, plant-parasitic nematodes like Columbia and Northern Root-knots, Stubby Root, Root Lesion, and Dagger nematodes, can make considerable negative impacts on quality and quantity of economically important crops including Potatoes, Onions, Alfalfa, Mint… also, Grapes and tree fruits like Apples, Cherries, Blueberries…

Nematode testing: The nematode assay lab in AGNEMA™ focuses on the identification of the frugally vital plant-parasitic nematodes in soil, water, and plant material samples. Through nematode assay, plant-pathogenic nematodes are identified and counted. Moreover, a nematode species that is the smallest group of populations can be characterized by a unique set of morphological or DNA genetic traits.

Please check guidelines for collecting samples.

Whether you are planning to prepare a field or manage an established field, keep controlling of plant-parasitic nematode population is a key to make informed decision and success.